Peter Parker/Spider-Man: Nicholas Hammond (1977-1981) | Tobey Maguire (2002-2007) | Andrew Garfield (2012-2014) | Tom Holland (2016-) | Jake Johnson (2018-)

May Parker: Jeff Donnell (1977) | Rosemary Harris (2002-2007) | Sally Field (2012-2014) | Marisa Tomei (2016-) | Lily Tomlin (2018-)

Gwen Stacy: Bryce Dallas Howard (2007) | Emma Stone (2012-2014) | Hailee Steinfeld (2018-)

Wilson Fisk/Kingpin: Michael Clarke Duncan (2003) | Vincent D'Onofrio (2015-2018) | Liev Schreiber (2018-)

Mary Jane Watson: Kirsten Dunst (2002-2007) | Zoë Kravitz (2018-)

Aaron Davis/Prowler: Donald Glover (2017-) | Mahershala Ali (2018)